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The TM-score program is to compare two models based on their given residue equivalency i. It is usually NOT applied to compare two proteins of different sequences. The TM-align is a structural alignment program for comparing two proteins whose sequences can be different.

The TM-align will first find the best equivalent residues of two proteins based on the structure similarity and then output a TM-score. The TM-score values in both programs have the same definition; however, the TM-score value reported by TM-align is usually higher than that reported by TM-score program due to the optimized alignment in TM-align. Why is TM-score different when normalized by different proteins? This is the reason that we need to specify which protein the TM-score is normalized by, when we report a TM-score value.

Be default, TM-score program reports the TM-score that is normalized by the length of the second protein. Update history please always download the newest version of TMscore.

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